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KMID : 1144820200260040275
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대한의생명과학회지
2020 Volume.26 No. 4 p.275 ~ p.287
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Validation of Reduced-volume Reaction in the PowerQuant® System for human DNA Quantification
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Kim Hyo-Jeong
Cho Yoon-Jung
Kim Jeong-Yong
Lee Ja-Hyun
Kim Hyo-Sook
Kim Eung-Soo
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Abstract
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Since its introduction in the forensic field, quantitative PCR (qPCR) has played an essential role in DNA analysis. Quality of DNA should be evaluated before short tandem repeat (STR) profiling to obtain reliable results and reduce unnecessary costs. To this end, various human DNA quantification kits have been developed. Among these kits, the PowerQunatⓡ System was designed not only to determine the total amount of human DNA and human male DNA from a forensic evidence item, but also to offer data about degradation of DNA samples. However, a crucial limitation of the PowerQunatⓡ System is its high cost. Therefore, to minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/2-volume) using DNA samples of varying types and concentrations. Our results demonstrated that the low-volume method has almost comparable performance to the manufacturer's method for human DNA quantification, human male DNA quantification, and DNA degradation index. Furthermore, using a reduced volume of regents, it is possible to run 2 times more reactions per kit. We expect the proposed low-volume method to cut costs in half for laboratories dealing with large numbers of DNA samples.
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KEYWORD
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Quantitative PCR, Short tandem repeat (STR) profiling, PowerQunat® System, Human DNA quantification kit
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